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Kristin Clothier   Dr.  Institute, Department or Faculty Head 
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Kristin Clothier published an article in April 2019.
Top co-authors See all
Lisa A. Tell

106 shared publications

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA

Luisa W. Cheng

46 shared publications

Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, USDA-ARS, 800 Buchanan St., Albany, CA 94710, USA

Kirkwood M. Land

37 shared publications

Department of Biological Sciences, University of the Pacific, 3601 Pacific Avenue, Stockton, CA 95211, USA

Barbara A. Byrne

15 shared publications

Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA

Jong H. Kim

2 shared publications

Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, USDA-ARS, 800 Buchanan St., Albany, CA 94710, USA

5
Publications
2
Reads
0
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9
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Publication Record
Distribution of Articles published per year 
(2016 - 2019)
Total number of journals
published in
 
5
 
Publications
Article 0 Reads 0 Citations High Efficiency Drug Repurposing Design for New Antifungal Agents Jong H. Kim, Kathleen L. Chan, Luisa W. Cheng, Lisa A. Tell,... Published: 17 April 2019
Methods and Protocols, doi: 10.3390/mps2020031
DOI See at publisher website ABS Show/hide abstract
Current antifungal interventions have often limited efficiency in treating fungal pathogens, particularly those resistant to commercial drugs or fungicides. Antifungal drug repurposing is an alternative intervention strategy, whereby new utility of various marketed, non-antifungal drugs could be repositioned as novel antifungal agents. In this study, we investigated “chemosensitization” as a method to improve the efficiency of antifungal drug repurposing, wherein combined application of a second compound (viz., chemosensitizer) with a conventional, non-antifungal drug could greatly enhance the antifungal activity of the co-applied drug. Redox-active natural compounds or structural derivatives, such as thymol (2-isopropyl-5-methylphenol), 4-isopropyl-3-methylphenol, or 3,5-dimethoxybenzaldehyde, could serve as potent chemosensitizers to enhance antifungal activity of the repurposed drug bithionol. Of note, inclusion of fungal mutants, such as antioxidant mutants, could also facilitate drug repurposing efficiency, which is reflected in the enhancement of antifungal efficacy of bithionol. Bithionol overcame antifungal (viz., fludioxonil) tolerance of the antioxidant mutants of the human/animal pathogen Aspergillus fumigatus. Altogether, our strategy can lead to the development of a high efficiency drug repurposing design, which enhances the susceptibility of pathogens to drugs, reduces time and costs for new antifungal development, and abates drug or fungicide resistance.
Article 0 Reads 1 Citation Frequency, serotype distribution, and antimicrobial susceptibility patterns of Salmonella in small poultry flocks in Cal... Kristin A. Clothier, Peony Kim, Aslı Mete, Ashley E. Hill Published: 06 February 2018
Journal of Veterinary Diagnostic Investigation, doi: 10.1177/1040638718755418
DOI See at publisher website
Article 2 Reads 1 Citation Characterization of Pajaroellobacter abortibovis , the etiologic agent of epizootic bovine abortion Roxann S. Brooks, Myra T. Blanchard, Kristin A. Clothier, Sc... Published: 01 August 2016
Veterinary Microbiology, doi: 10.1016/j.vetmic.2016.07.001
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Highlights•The agent of epizootic bovine abortion has been named Pajaroellobacter abortibovis.•Gram stain and electron microscopy confirm P. abortibovis to be a gram negative rod.•Flow cytometric analysis establishes the intracellular presence of P. abortibovis in leukocytes.•Pajaroellobacter abortibovis partial gene sequences were established for 23 S rRNA, fusA and pyrG.•Phylogenic analysis places P. abortibovis in a genus unique from Sorangium. AbstractEpizootic bovine abortion (EBA), first identified in the 1950s, is a major contributor of economic loss to western U.S. beef producers. The causative agent proved elusive for over fifty years until a novel Deltaproteobacteria was identified as the etiologic agent in 2005. The microbe, which has yet to be successfully cultured in vitro, has proven difficult to purify from necropsy tissues. Thus, phylogenetic characterization has been limited to analysis of the 16 S ribosomal RNA (rRNA) gene (AF503916), which placed this bacterium in the order Myxococcales, suborder Sorangiineae, family Polyangiaceae and most closely related to Sorangium cellulosum. The focus of the current study was to further expand the morphologic characterization and taxonomic placement of this bacteria, named here as Pajaroellobacter abortibovis. Modified Gram staining, combined with transmission electron microscopy, provide strong evidence that the bacterium is gram negative. Flow cytometric analysis identified the presence of P. abortibovis in murine leukocytes. While attempts to sequence ten universally conserved protein-coding genes using previously published degenerative primers failed, redesigned primers based solely upon Deltaproteobacteria facilitated the partial sequencing of two genes; fusA (JQ173112) and pyrG (JQ173111). Primers designed in a similar fashion generated a partial sequence of the 23 S rRNA gene (JQ173113) These sequences, combined with a revised 16 S rRNA phylogenic analysis, support the placement of this bacteria as a unique genus separate from Sorangium.
Article 0 Reads 5 Citations Evaluation of bovine abortion cases and tissue suitability for identification of infectious agents in California diagnos... K. Clothier, M. Anderson, Press Enter Key For Correspondence... Published: 01 March 2016
Theriogenology, doi: 10.1016/j.theriogenology.2015.11.001
DOI See at publisher website PubMed View at PubMed
Article 0 Reads 2 Citations Phenotypic and Genotypic Characterization of Animal-Source Salmonella Heidelberg Isolates Kristin A. Clothier, Barbara A. Byrne Published: 03 January 2016
Journal of Veterinary Medicine, doi: 10.1155/2016/6380890
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Salmonella enterica serotype Heidelberg (S. Heidelberg) is frequently implicated in human foodborne Salmonella infections and often produces more severe clinical disease than other serotypes. Livestock and poultry products represent a potential risk for transmission to humans. The purpose of this study was to evaluate 49 S. Heidelberg veterinary isolates for exponential growth rate (EGR), PFGE pattern, and antimicrobial resistance to evaluate these parameters as mechanisms by which S. Heidelberg emerged as a virulent foodborne pathogen. Isolates were categorized by species of origin; clinical or environmental sources; and time frame of recovery. Growth rates were determined in nutrient media using serial dilutions and colony counts; PFGE was performed according to the CDC PulseNet protocol. Minimum inhibitory concentration and susceptibility determinations were performed against antimicrobials important in human medicine. Eighteen unique PFGE patterns were detected in the isolates tested. Antimicrobial resistance was significantly greater (P < 0.05) for ten of 15 drugs in clinical over environmental isolates; for four drugs between the time frames; and for ten drugs between species of origin. The large genetic diversity present in isolates of this serotype may convey competitive advantages to this organism, while the presence of antimicrobial resistance represents a potential zoonotic risk via animal-source food products.
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