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Luisa Cheng   Dr.  Research or Laboratory Scientist 
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Luisa Cheng published an article in April 2019.
Top co-authors See all
Mendel Friedman

348 shared publications

Healthy Processed Foods Research, Western Regional Research Center, Agricultural Research Service, United States Dept. of Agriculture, Albany, CA, 94710, U.S.A.

Peter J. Sadler

211 shared publications

Department of Chemistry; University of Warwick; Coventry CV4 7AL UK

Michel R. Popoff

200 shared publications

Bacterial Toxins, Institut Pasteur, ERL 6002, 75015 Paris, France

Vicky M. Avery

188 shared publications

Eskitis Institute for Drug DiscoveryGriffith University Brisbane Innovation Park Campus Nathan QLD 4111 Australia

Hannu Korkeala

135 shared publications

Department of Food Hygiene and Environmental Health, University of Helsinki, P.O. Box 66, FI-00014 Helsinki, Finland;(Y.D.);(K.S.);(H.K.)

45
Publications
38
Reads
7
Downloads
213
Citations
Publication Record
Distribution of Articles published per year 
(2008 - 2019)
Total number of journals
published in
 
21
 
Publications See all
Article 0 Reads 0 Citations High Efficiency Drug Repurposing Design for New Antifungal Agents Jong H. Kim, Kathleen L. Chan, Luisa W. Cheng, Lisa A. Tell,... Published: 17 April 2019
Methods and Protocols, doi: 10.3390/mps2020031
DOI See at publisher website ABS Show/hide abstract
Current antifungal interventions have often limited efficiency in treating fungal pathogens, particularly those resistant to commercial drugs or fungicides. Antifungal drug repurposing is an alternative intervention strategy, whereby new utility of various marketed, non-antifungal drugs could be repositioned as novel antifungal agents. In this study, we investigated “chemosensitization” as a method to improve the efficiency of antifungal drug repurposing, wherein combined application of a second compound (viz., chemosensitizer) with a conventional, non-antifungal drug could greatly enhance the antifungal activity of the co-applied drug. Redox-active natural compounds or structural derivatives, such as thymol (2-isopropyl-5-methylphenol), 4-isopropyl-3-methylphenol, or 3,5-dimethoxybenzaldehyde, could serve as potent chemosensitizers to enhance antifungal activity of the repurposed drug bithionol. Of note, inclusion of fungal mutants, such as antioxidant mutants, could also facilitate drug repurposing efficiency, which is reflected in the enhancement of antifungal efficacy of bithionol. Bithionol overcame antifungal (viz., fludioxonil) tolerance of the antioxidant mutants of the human/animal pathogen Aspergillus fumigatus. Altogether, our strategy can lead to the development of a high efficiency drug repurposing design, which enhances the susceptibility of pathogens to drugs, reduces time and costs for new antifungal development, and abates drug or fungicide resistance.
Article 0 Reads 0 Citations Influence of Food Matrices on the Stability and Bioavailability of Abrin Christina C. Tam, Thomas D. Henderson, Larry H. Stanker, Lui... Published: 01 December 2018
Toxins, doi: 10.3390/toxins10120502
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Abrin, a highly toxic plant toxin, is a potential bioterror weapon. Work from our laboratory and others have shown that abrin is highly resistant to both thermal and pH inactivation methods. We sought to evaluate the effectiveness of selected food processing thermal inactivation conditions against abrin in economically important food matrices (whole milk, non-fat milk, liquid egg, and ground beef). The effectiveness of toxin inactivation was measured via three different assays: (1) In vitro cell free translation (CFT) assay, (2) Vero cell culture cytotoxicity; and the in vivo mouse intraperitoneal (ip) bioassay. For both whole and non-fat milk, complete inactivation was achieved at temperatures of ≥80 °C for 3 min or 134 °C for 60 s, which were higher than the normal vat/batch pasteurization or the high temperature short time pasteurization (HTST). Toxin inactivation in liquid egg required temperatures of ≥74 °C for 3 min higher than suggested temperatures for scrambled eggs (22% solids) and plain whole egg. Additionally, the ground beef (80:20%) matrix was found to be inhibitory for full toxin activity in the mouse bioassay while retaining some activity in both the cell free translation assay and Vero cell culture cytotoxicity assay.
Article 0 Reads 0 Citations A Rapid, Sensitive, and Portable Biosensor Assay for the Detection of Botulinum Neurotoxin Serotype A in Complex Food Ma... Christina C. Tam, Andrew R. Flannery, Luisa W. Cheng Published: 15 November 2018
Toxins, doi: 10.3390/toxins10110476
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Botulinum neurotoxin (BoNT) intoxication can lead to the disease botulism, characterized by flaccid muscle paralysis that can cause respiratory failure and death. Due to the significant morbidity and mortality costs associated with BoNTs high toxicity, developing highly sensitive, rapid, and field-deployable assays are critically important to protect the nation’s food supply against either accidental or intentional contamination. We report here that the B-cell based biosensor assay CANARY® (Cellular Analysis and Notification of Antigen Risks and Yields) Zephyr detects BoNT/A holotoxin at limits of detection (LOD) of 10.0 ± 2.5 ng/mL in assay buffer. Milk matrices (whole milk, 2% milk and non-fat milk) with BoNT/A holotoxin were detected at similar levels (7.4–7.9 ng/mL). BoNT/A complex was positive in carrot, orange, and apple juices at LODs of 32.5–75.0 ng/mL. The detection of BoNT/A complex in solid complex foods (ground beef, smoked salmon, green bean baby puree) ranged from 14.8 ng/mL to 62.5 ng/mL. Detection of BoNT/A complex in the viscous liquid egg matrix required dilution in assay buffer and gave a LOD of 171.9 ± 64.7 ng/mL. These results show that the CANARY® Zephyr assay can be a highly useful qualitative tool in environmental and food safety surveillance programs.
Article 1 Read 0 Citations Cinnamoyl-Oxaborole Amides: Synthesis and Their in Vitro Biological Activity Maureen Gumbo, Richard M. Beteck, Tawanda Mandizvo, Ronnett ... Published: 15 August 2018
Molecules, doi: 10.3390/molecules23082038
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Due to the increased interest in their application in the treatment of infectious diseases, boron-containing compounds have received a significant coverage in the literature. Herein, a small set of novel cinnamoly-oxaborole amides were synthesized and screened against nagana Trypanosoma brucei brucei for antitrypanosomal activity. Compound 5g emerged as a new hit with an in vitro IC50 value of 0.086 μM against T. b. brucei without obvious inhibitory activity against HeLa cell lines. The same series was also screened against other human pathogens, including Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), for which moderate to weak activity (10 to >125 μM) was observed. Similarly, these compounds exhibited moderate activity against the human protozoal pathogen Trichomonas vaginalis with no observed effect on common microbiome bacterial species. The cross-species inhibitory activity presents the possibility of these compounds serving as broad-spectrum antibiotics for these prevalent three human pathogens.
Article 0 Reads 1 Citation Potato Peels and Their Bioactive Glycoalkaloids and Phenolic Compounds Inhibit the Growth of Pathogenic Trichomonads Mendel Friedman, Vincent Huang, Quincel Quiambao, Sabrina No... Published: 24 July 2018
Journal of Agricultural and Food Chemistry, doi: 10.1021/acs.jafc.8b01726
DOI See at publisher website
Article 0 Reads 0 Citations Frontispiece: Organometallic Conjugates of the Drug Sulfadoxine for Combatting Antimicrobial Resistance Prinessa Chellan, Vicky M. Avery, Sandra Duffy, James A. Tri... Published: 17 July 2018
Chemistry – A European Journal, doi: 10.1002/chem.201884064
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