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Guangshun Wang   Professor  Institute, Department or Faculty Head 
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Guangshun Wang published an article in August 2018.
Research Keywords & Expertise See all
0 A
0 Antiviral
0 Innate Immunity
0 NMR Spectroscopy
0 Structural
0 antimicrobial peptide
Top co-authors See all
Mark A. Baker

81 shared publications

Priority Research Centre in Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia

Nongnuj Tanphaichitr

59 shared publications

Department of Obstetrics and GynecologyFaculty of Medicine, University of Ottawa, Ottawa, Canada

Biswajit Mishra

21 shared publications

Department of Pathology and Microbiology; College of Medicine; University of Nebraska Medical Center; 985900 Nebraska Medical Center Omaha NE 68198-5900 USA

Weihua Li

3 shared publications

Key Laboratory of Reproduction Regulation of National Population and Family Planning Commission, The Shanghai Institute of Planned Parenthood Research, Institute of Reproduction and Development, Fudan University, Shanghai 200032, China

Duane Hickling

1 shared publications

Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, Ontario K1H 8L6, ON, Canada.

40
Publications
23
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0
Downloads
877
Citations
Publication Record
Distribution of Articles published per year 
(2002 - 2018)
Total number of journals
published in
 
24
 
Publications See all
Article 0 Reads 1 Citation Antibacterial, antifungal, anticancer activities and structural bioinformatics analysis of six naturally occurring tempo... Biswajit Mishra, Xiuqing Wang, Tamara Lushnikova, Yingxia Zh... Published: 01 August 2018
Peptides, doi: 10.1016/j.peptides.2018.05.011
DOI See at publisher website
Article 3 Reads 1 Citation Amino Acid Composition Determines Peptide Activity Spectrum and Hot-Spot-Based Design of Merecidin Xiuqing Wang, Biswajit Mishra, Tamara Lushnikova, Jayaram La... Published: 26 March 2018
Advanced Biosystems, doi: 10.1002/adbi.201700259
DOI See at publisher website
Article 2 Reads 5 Citations Arginine-lysine positional swap of the LL-37 peptides reveals evolutional advantages of the native sequence and leads to... Xiuqing Wang, José Carlos Bozelli Junior, Biswajit Mishra, T... Published: 01 August 2017
Biochimica et Biophysica Acta (BBA) - Biomembranes, doi: 10.1016/j.bbamem.2017.04.018
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Antimicrobial peptides are essential components of the innate immune system of multicellular organisms. Although cationic and hydrophobic amino acids are known determinants of these amphipathic molecules for bacterial killing, it is not clear how lysine-arginine (K-R) positional swaps influence peptide structure and activity. This study addresses this question by investigating two groups of peptides (GF-17 and 17BIPHE2) derived from human cathelicidin LL-37. K-R positional swap showed little effect on minimal inhibitory concentrations of the peptides. However, there are clear differences in bacterial killing kinetics. The membrane permeation patterns vary with peptide and bacterial types, but not changes in fluorescent dyes, salts or pH. In general, the original peptide is more efficient in bacterial killing, but less toxic to human cells, than the K-R swapped peptides, revealing the evolutionary significance of the native sequence for host defense. The characteristic membrane permeation patterns for different bacteria suggest a possible application of these K-R positional-swapped peptides as molecular probes for the type of bacteria. Such differences are related to bacterial membrane compositions: minimal for Gram-positive Staphylococcus aureus with essentially all anionic lipids (cardiolipin and phosphatidylglycerol), but evident for Gram-negative Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli with a mixture of phosphatidylethanolamine and phosphatidylglycerol. Biophysical characterization found similar structures and binding affinities for these peptides in vesicle systems mimicking E. coli and S. aureus. It seems that interfacial arginines of GF-17 are preferred over lysines in bacterial membrane permeation. Our study sheds new light on the design of cationic amphipathic peptides.
Article 0 Reads 3 Citations The π Configuration of the WWW Motif of a Short Trp-Rich Peptide Is Critical for Targeting Bacterial Membranes, Disrupti... D. Zarena, Biswajit Mishra, Tamara Lushnikova, Fangyu Wang, ... Published: 26 July 2017
Biochemistry, doi: 10.1021/acs.biochem.7b00456
DOI See at publisher website PubMed View at PubMed
Article 2 Reads 2 Citations Titanium surfaces immobilized with the major antimicrobial fragment FK-16 of human cathelicidin LL-37 are potent against... Biswajit Mishra, Guangshun Wang Published: 04 July 2017
Biofouling, doi: 10.1080/08927014.2017.1332186
DOI See at publisher website PubMed View at PubMed
Article 0 Reads 7 Citations Individual and Combined Effects of Engineered Peptides and Antibiotics on Pseudomonas aeruginosa Biofilms Biswajit Mishra, Guangshun Wang Published: 25 June 2017
Pharmaceuticals, doi: 10.3390/ph10030058
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Pseudomonas aeruginosa is involved in a variety of difficult-to-treat infections frequently due to biofilm formation. To identify useful antibiofilm strategies, this article evaluated efficacy of two newly engineered cationic antimicrobial peptides (17BIPHE2 and DASamP2), traditional antibiotics, and their combinations against biofilms at different stages. 17BIPHE2 is designed based on the 3D structure of human cathelicidin LL-37 and DASamP2 is derived from database screening. While both peptides show effects on bacterial adhesion, biofilm formation, and preformed biofilms, select antibiotics only inhibit biofilm formation, probably due to direct bacterial killing. In addition, the time dependence of biofilm formation and treatment in a static in vitro biofilm model was also studied. The initial bacterial inoculum determines the peptide concentration needed to inhibit biofilm growth. When the bacterial growth time is less than 8 h, the biomass in the wells can be dispersed by either antibiotics alone or peptides alone. However, nearly complete biofilm disruption can be achieved when both the peptide and antibiotics are applied. Our results emphasize the importance of antibiofilm peptides, early treatment using monotherapy, and the combination therapy for already formed biofilms of P. aeruginosa.
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